Difference between revisions of "Part:BBa K1893021"
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This is the Anti-STAR sequence under control of the Las quorum signalling system. The gene encoding the transcriptional activator LasR is downstream a constitutive Anderson promoter, j23101. LasR is activated by 3O-C12-HSL. Included in the construct is the LasR-activated promoter pLas. | This is the Anti-STAR sequence under control of the Las quorum signalling system. The gene encoding the transcriptional activator LasR is downstream a constitutive Anderson promoter, j23101. LasR is activated by 3O-C12-HSL. Included in the construct is the LasR-activated promoter pLas. | ||
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Latest revision as of 14:44, 23 October 2016
Las receiver with anti-STAR (LasR+pLas+anti-STAR)
This is the Anti-STAR sequence under control of the Las quorum signalling system. The gene encoding the transcriptional activator LasR is downstream a constitutive Anderson promoter, j23101. LasR is activated by 3O-C12-HSL. Included in the construct is the LasR-activated promoter pLas.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]