Difference between revisions of "Part:BBa K1893018"
Line 5: Line 5:
 
Stuff
 
Stuff
  
−
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 14:24, 23 October 2016


Arabinose inducible 3-isopropylmalate dehydrogenase (pBAD+leuB)

Stuff


Usage and Biology

The leucine biosynthesis pathway is activated in conditions of leucine starvation and can be manipulated for the purposes of auxotrophy experiments. We initially considered auxotrophy as a method of controlling growth through our growth-regulation module. This part was designed to investigate the effect of leuB suppression on the growth of E. coli leuB knockout strains. The knockout strains were to be grown in minimal media lacking leucine, so that only cells expressing leuB at a sufficient level would be capable of growth. Due to time constraints, and the difficulty of creating knockout strains, we were unable to obtain any characterisation data.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098