Difference between revisions of "Part:BBa K1893017"
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A GFP reporter under control of a pBAD promoter, used to characterise the activation range of the pBAD promoter
 
A GFP reporter under control of a pBAD promoter, used to characterise the activation range of the pBAD promoter
  
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===Usage and Biology===
 
===Usage and Biology===
 
Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of GFP.  
 
Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of GFP.  
  
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We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded flourescence in a BMG plate reader.  
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We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1893017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893017 SequenceAndFeatures</partinfo>

Revision as of 14:24, 23 October 2016


Arabinose inducible GFP (pBAD+GFP)

A GFP reporter under control of a pBAD promoter, used to characterise the activation range of the pBAD promoter

Usage and Biology

Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of GFP.

We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2017
    Illegal SapI site found at 1098