Difference between revisions of "Part:BBa K1893018"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The leucine biosynthesis pathway is activated in conditions of leucine starvation and can be manipulated for the purposes of auxotrophy experiments. We initially considered auxotrophy as a method of controlling growth through our growth-regulation module. This part was designed to investigate the effect of leuB suppression on the growth of E. coli leuB knockout strains. The knockout strains were to be grown in minimal media lacking leucine, so that only cells expressing leuB at a sufficient level would be capable of growth. Due to time constraints, and the difficulty of creating knockout strains, we were unable to obtain any characterisation data. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1893018 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1893018 SequenceAndFeatures</partinfo> | ||
Revision as of 13:49, 23 October 2016
Arabinose inducible 3-isopropylmalate dehydrogenase (pBAD+leuB)
Stuff