Difference between revisions of "Part:BBa K1963004"

Revision as of 13:24, 23 October 2016

An sRNA system for down-regulation of E. coli fliC encoding flagellin.

This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq BBa_K1963000. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed betweeen C-193 and T-194. The system should impair motility of E. coli.

Usage and Biology

The motile E. coli host strain MG1655 was tranformed with BBa_K1963004 and subjected to a plate-based swimming assay (Figure 1). The BBa_K1963004 contains a sRNA that will target the initial codons of the fliC transcript that encodes flagellin - the principle component of the flagellum. The data related to this biobrick are labelled spiRNA-fliC-CDS in Figure 1.

experimental procedure needed.

Figure 1. sRNA Devices can Inhibit Swimming in an E. coli Test System. Photographs representative of motility assays with MG1655 transformed with BBa_K1963002, BBa_K1963004 and BBa_K1963010. Bar graph showing average swimming/spreading area and standard deviation of replicates.

Sequence and Features