Difference between revisions of "Part:BBa K1893014:Design"
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Prefix Reverse primer: CTCTAGAAGCGGCCGCG
 
Prefix Reverse primer: CTCTAGAAGCGGCCGCG
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===Source===
 
===Source===

Revision as of 13:21, 23 October 2016


anti-STAR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was obtained through the use of inverse PCR on the 'j23119-AntiSTAR/pSB1C3' construct (BBa_K1893011) to remove the constitutive promoter j23119.

Anti-STAR forward primer: AGTTTTTACAGTGAATTGTTTTAATTAGTTG

Prefix Reverse primer: CTCTAGAAGCGGCCGCG

Source

Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time.

References

Imperial iGEM 2016