Difference between revisions of "Part:BBa K1893009"

 
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<partinfo>BBa_K1893009 short</partinfo>
 
<partinfo>BBa_K1893009 short</partinfo>
  
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Stuff
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This is the STAR repoter plasmid containing the pAD1.S5 attenuator sequence upstream to the reporter gene SFGFP. Transcription of SFGFP is also controlled by constitutive promoter j23119 and terminator TrrnB.
  
 
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Revision as of 10:53, 23 October 2016


STAR inducible superfolder GFP (pAD1.S5+SF-GFP)

This is the STAR repoter plasmid containing the pAD1.S5 attenuator sequence upstream to the reporter gene SFGFP. Transcription of SFGFP is also controlled by constitutive promoter j23119 and terminator TrrnB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 959
    Illegal BsaI site found at 1137