Difference between revisions of "Part:BBa K1961006"
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HSiTAIWAN iGEM16
  
  

Revision as of 08:05, 23 October 2016


Lead ion detector

This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+ - PbrR in the presence of Pb2+ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.


We redo ligation and do function assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 686

HSiTAIWAN_DESCRIPTION_upload_K1613017.jpeg HSiTAIWAN iGEM16