Difference between revisions of "Part:BBa K1961006"
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(1) PbrR generation:this part produces PbrR which can form a dimer of Pb<sup>2+</sup> - PbrR in the presence of Pb<sup>2+</sup> . | (1) PbrR generation:this part produces PbrR which can form a dimer of Pb<sup>2+</sup> - PbrR in the presence of Pb<sup>2+</sup> . | ||
(2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb<sup>2+</sup> /PbrR. RFP expression can be observed when the dimer binds onto the promoter. | (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb<sup>2+</sup> /PbrR. RFP expression can be observed when the dimer binds onto the promoter. | ||
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| + | We redo ligation and do function assay. | ||
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Revision as of 08:05, 23 October 2016
Lead ion detector
This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+ - PbrR in the presence of Pb2+ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.
We redo ligation and do function assay.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1281
Illegal AgeI site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 686
