Difference between revisions of "Part:BBa K1961005:Design"

 
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===Design Notes===
 
===Design Notes===
 
PbrR is a Pb2+ binding protein.  In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).  We compared their GFP expression    to measure their PbrR generation.
 
PbrR is a Pb2+ binding protein.  In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).  We compared their GFP expression    to measure their PbrR generation.
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[[File: HSiTAIWAN_WIKI_K1961005_map_1.jpeg|500px|thumb|left|alt text]]
  
  

Revision as of 07:22, 23 October 2016


PbrR generator expressed by GFP under control of general promoter (J23102)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111


Design Notes

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation.

alt text


Source

We used parts from iGEM stocks.

References