Difference between revisions of "Part:BBa K1961004"
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The Pb<sup>2+</sup> biosensor involves (1) generation of PbrR promoter with a binding site for lead ion, thus forming Pb2+-PbrR dimer and (2) GFP expression when Pb2+-PbrR dimer binds onto pbr promoter. Because the PbrR and Pb<sup>2+</sup> - PbrR dimer compete to bind onto pbr promoters and result in poor GFP performance, we designed an autoregulated PbrR generator (BBa_K1961004) to control the amount of PbrR. This composite part consists of the TetR and PbrR generators, both controlled by P<sub>Tet</sub>, which has TetR binding sites. TetRs bind onto P<sub>Tet</sub> and repress the gene expression of TetR, PbrR and GFP. We used tetracycline to induce the gene expression of PbrR and GFP. | The Pb<sup>2+</sup> biosensor involves (1) generation of PbrR promoter with a binding site for lead ion, thus forming Pb2+-PbrR dimer and (2) GFP expression when Pb2+-PbrR dimer binds onto pbr promoter. Because the PbrR and Pb<sup>2+</sup> - PbrR dimer compete to bind onto pbr promoters and result in poor GFP performance, we designed an autoregulated PbrR generator (BBa_K1961004) to control the amount of PbrR. This composite part consists of the TetR and PbrR generators, both controlled by P<sub>Tet</sub>, which has TetR binding sites. TetRs bind onto P<sub>Tet</sub> and repress the gene expression of TetR, PbrR and GFP. We used tetracycline to induce the gene expression of PbrR and GFP. | ||
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[[File: HSiTAIWAN_WIKI_K1961004_auto_circuit.jpeg|300px|thumb|left|alt text]] | [[File: HSiTAIWAN_WIKI_K1961004_auto_circuit.jpeg|300px|thumb|left|alt text]] | ||
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[[File: HSiTAIWAN_WIKI_K1961004_auto_circuit.jpeg|300px|thumb|left|alt text]] | [[File: HSiTAIWAN_WIKI_K1961004_auto_circuit.jpeg|300px|thumb|left|alt text]] | ||
+ | Figure 1. The PbrR generation is repressed by P<sub>Tet</sub>, which has TetR binding sites | ||
− | + | Figure 2. Use tetracycline to induce the PbrR generation and GFP expression | |
Revision as of 07:01, 23 October 2016
PbrR generator expressed by GFP under autoregulation of PTet
The Pb2+ biosensor involves (1) generation of PbrR promoter with a binding site for lead ion, thus forming Pb2+-PbrR dimer and (2) GFP expression when Pb2+-PbrR dimer binds onto pbr promoter. Because the PbrR and Pb2+ - PbrR dimer compete to bind onto pbr promoters and result in poor GFP performance, we designed an autoregulated PbrR generator (BBa_K1961004) to control the amount of PbrR. This composite part consists of the TetR and PbrR generators, both controlled by PTet, which has TetR binding sites. TetRs bind onto PTet and repress the gene expression of TetR, PbrR and GFP. We used tetracycline to induce the gene expression of PbrR and GFP.
Figure 1. The PbrR generation is repressed by PTet, which has TetR binding sites
Figure 2. Use tetracycline to induce the PbrR generation and GFP expression
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2040