Difference between revisions of "Part:BBa K1961004:Design"

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===Design Notes===
 
===Design Notes===
 
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet.  We used tetracycline to induce the transcription.
 
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet.  We used tetracycline to induce the transcription.
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[[File:[[File:File.png|400px|thumb|left|alt text]] |200px|thumb|left|alt text]]
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[[File:https://static.igem.org/mediawiki/parts/9/93/HSiTAIWAN_WIKI_K1961004_auto_map.png|400px|thumb|left|alt text]]
  
 
===Source===
 
===Source===

Revision as of 06:40, 23 October 2016


PbrR generator expressed by GFP under autoregulation of PTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2040


Design Notes

This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription.


File:Https://static.igem.org/mediawiki/parts/9/93/HSiTAIWAN WIKI K1961004 auto map.png
alt text

Source

We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012

References