Difference between revisions of "Part:BBa K1961004:Design"
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===Design Notes=== | ===Design Notes=== | ||
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription. | This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription. | ||
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+ | [[File:[[File:File.png|400px|thumb|left|alt text]] |200px|thumb|left|alt text]] | ||
===Source=== | ===Source=== |
Revision as of 06:38, 23 October 2016
PbrR generator expressed by GFP under autoregulation of PTet
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2040
Design Notes
This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription.
[[File: |200px|thumb|left|alt text]]Source
We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012