Difference between revisions of "Part:BBa K2100013"

Latest revision as of 03:58, 23 October 2016

pEXPR hEF1a-tagBFP

tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. This cascade can be used as a transfection marker to determine your efficiency for many experiments in mammalian synbio.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal BglII site found at 591
Illegal BamHI site found at 1197
Illegal BamHI site found at 1227
Illegal XhoI site found at 990
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
Illegal NgoMIV site found at 725
Illegal AgeI site found at 103
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2100013 short</partinfo>
-tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter.
+tagBFP is a blue fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. This cascade can be used as a transfection marker to determine your efficiency for many experiments in mammalian synbio.
 <!-- Add more about the biology of this part here