Difference between revisions of "Part:BBa K1962006"

Revision as of 23:39, 22 October 2016

Truncated Colicin E9 Lacking Bacteriocin Active Domain

Colicins are anti-bacterial proteins produced by some strains of E. coli that typically have three domains: a translocation domain; a receptor binding domain; and a cytotoxic domain. This biobrick encodes a truncated version of Colicin E9 which lacks the C-terminal bacterocin domain, which is this case is a DNase.

The part has an engineered multiple cloning site at the 3' end, preceding the RFC[10] suffix and regulation double stop codons, containing the following in-frame restriction sites: BamHI, KpnI, SalI, BglII and NheI. The presence of this multiple cloning site will allow for different toxic domains, or other polypeptides, to be fused at the C-terminus of the truncated colicin and thereby generating a synthetic colicin or novel fusion protein in a rapid and facile manner.

This Biobrick was used to generate two synthetic colicins (BBa_K1962007 and BBa_K1962008).

Sequence and Features