Difference between revisions of "Part:BBa K1963010"

Revision as of 22:49, 22 October 2016

An sRNA system for down-regulation of E. coli fliC encoding flagellin

This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq BBa_K1963000. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the RBS and 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed after C-193. The system should impair motility of E. coli.

Usage and Biology

Experimental procedures needed.

Figure 1. Bar graph showing average colony area and standard deviation of replicates. Photographs representative of motility assays with BBa_K1963002, BBa_K1963004 and BBa_K1963010.

Sequence and Features