Difference between revisions of "Part:BBa K1962015"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We cloned the Ia-Immunity protein (<partinfo>BBa_K1962001</partinfo>) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (BBa_K1962011), respectively. | + | We cloned the Ia-Immunity protein (<partinfo>BBa_K1962001</partinfo>) downstream of a pH sensitive promoter Pasr (<partinfo>BBa_K1231000</partinfo>) and a bile salt sensitive promoter PacrRA (<partinfo>BBa_K1231001</partinfo>) to generate the composite parts (<partinfo>BBa_K1962015</partinfo>) and (<partinfo>BBa_K1962011</partinfo), respectively. |
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Revision as of 21:44, 22 October 2016
A device for expression of Im-Ia in response to pH
This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (asr from biobrick BBa_K1231000, an RBS (BBa_B0030) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (BBa_K1962001).
Usage and Biology
We cloned the Ia-Immunity protein (BBa_K1962001) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (No part name specified with partinfo tag.