Difference between revisions of "Part:BBa K1962015"

Revision as of 21:43, 22 October 2016

A device for expression of Im-Ia in response to pH

This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (asr from biobrick BBa_K1231000, an RBS (BBa_B0030) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (BBa_K1962001).

Usage and Biology

We cloned the Ia-Immunity protein (BBa_K1962001) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (BBa_K1962011), respectively.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 215
Illegal AgeI site found at 422
1000
COMPATIBLE WITH RFC[1000]

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 This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (<i>asr</i> from biobrick <partinfo>BBa_K1231000</partinfo>, an RBS (<partinfo>BBa_B0030</partinfo>) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (<partinfo>BBa_K1962001</partinfo>).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We cloned the Ia-Immunity protein (<partinfo>BBa_K1962001</partinfo>) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (BBa_K1962011), respectively.
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