Difference between revisions of "Part:BBa K1983017:Design"
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===Source=== | ===Source=== | ||
− | + | This part is derived from ([https://parts.igem.org/Part:BBa_B0017 BBa_B0017]). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies. | |
===References=== | ===References=== |
Latest revision as of 21:41, 22 October 2016
PheP under constitutive promoter, medium strength RBS and terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 98 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Primers used for cloning a medium strength RBS:
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC
RBS-Med: GCATCTAGAGACTCTAATCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG
Source
This part is derived from (BBa_B0017). Other parts are derived Escherichia coli and were synthesized by Integrate DNA Technologies.