Difference between revisions of "Part:BBa K1983013:Design"
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===Source=== | ===Source=== | ||
− | + | This part is derived from Escherichia coli and was synthesized by Integrate DNA Technologies. | |
===References=== | ===References=== |
Latest revision as of 21:32, 22 October 2016
Phenylalanine-specific permease (PheP) from Escherichia coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 37
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Source
This part is derived from Escherichia coli and was synthesized by Integrate DNA Technologies.