Difference between revisions of "Part:BBa K1893005:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Lots of stuff
+
This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pLux promoter which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the LuxR system
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
More Stuff
+
All parts used came from the iGEM 2016 DNA distribution
  
 
===References===
 
===References===

Latest revision as of 19:26, 22 October 2016


Lux receiver with GFP (LuxR+pLux+GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1712


Design Notes

This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pLux promoter which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the LuxR system


Source

All parts used came from the iGEM 2016 DNA distribution

References