Difference between revisions of "Part:BBa K1893004:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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Lots of stuff
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This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter.
  
  
  
 
===Source===
 
===Source===
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All parts came from the iGEM 2016 DNA distribution.
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More Stuff
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===References===
 
===References===

Latest revision as of 19:24, 22 October 2016


Lux receiver (LuxR+pLux)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter.


Source

All parts came from the iGEM 2016 DNA distribution.

References