Difference between revisions of "Part:BBa K1962007"

 
Line 5: Line 5:
 
This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (<partinfo>BBa_K1962006</partinfo>) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.
 
This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (<partinfo>BBa_K1962006</partinfo>) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.
  
−
<!-- Add more about the biology of this part here
+
 
−
===Usage and Biology===
+
Usage and Biology
 +
 
 +
https://static.igem.org/mediawiki/2016/a/af/T--Dundee--ResultsMid.png
  
 
<!-- -->
 
<!-- -->

Revision as of 18:47, 22 October 2016


Colicin E9::Ssp1 Chimera

This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (BBa_K1962006) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.


Usage and Biology

T--Dundee--ResultsMid.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1543
  • 1000
    COMPATIBLE WITH RFC[1000]