Difference between revisions of "Part:BBa I746352:Design"
m |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
The part does contain a RBS (B0034) already. | The part does contain a RBS (B0034) already. | ||
| + | |||
At position 84 of the coding region a silent point mutation was introduced (g==>c) to remove a PstI restriction site. | At position 84 of the coding region a silent point mutation was introduced (g==>c) to remove a PstI restriction site. | ||
Latest revision as of 12:07, 12 September 2007
delta activator from phiR73 phage
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part does contain a RBS (B0034) already.
At position 84 of the coding region a silent point mutation was introduced (g==>c) to remove a PstI restriction site.
Source
plasmid DNA supplied by Prof. Richard Calendar, University of California.