Difference between revisions of "Part:BBa K1983015:Design"
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This part has an XbaI site between RBS ([https://parts.igem.org/Part:BBa_J61132 J61132]) and PheP gene ([https://parts.igem.org/Part:BBa_K1983013 K1983013]) because it was created by fusing ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) with ([https://parts.igem.org/Part:BBa_K1983011 K1983011]). <br> | This part has an XbaI site between RBS ([https://parts.igem.org/Part:BBa_J61132 J61132]) and PheP gene ([https://parts.igem.org/Part:BBa_K1983013 K1983013]) because it was created by fusing ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) with ([https://parts.igem.org/Part:BBa_K1983011 K1983011]). <br> | ||
− | Since one of the fused parts is a composite biobrick ([https://parts.igem.org/Part: | + | Since one of the fused parts is a composite biobrick ([https://parts.igem.org/Part:BBa_K1983014 K1983014]) comprised of a promoter, RBS, coding gene and terminator, with XbaI it is more adaptable regarding the regulation of this gene. In addition, having only an XbaI site in the resulting fused part ([https://parts.igem.org/Part:BBa_K1983015 K1983015]), it can be still be combined with other biobricks when digested with SpeI and PstI. |
===Primers=== | ===Primers=== |
Revision as of 10:22, 22 October 2016
PheP and tRNA-Phe under constitutive promoters
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 98
Illegal NheI site found at 1669
Illegal NheI site found at 1692 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has an XbaI site between RBS (J61132) and PheP gene (K1983013) because it was created by fusing (K1983014) with (K1983011).
Since one of the fused parts is a composite biobrick (K1983014) comprised of a promoter, RBS, coding gene and terminator, with XbaI it is more adaptable regarding the regulation of this gene. In addition, having only an XbaI site in the resulting fused part (K1983015), it can be still be combined with other biobricks when digested with SpeI and PstI.
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Source
BBa_K1983015