Difference between revisions of "Part:BBa K1976020"
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        <h1><i>O</i>-Methyl-<i>L</i>-tyrosine tRNA synthetase </h1>
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This Part encodes the tRNA for nonnatural aminoacid <i>O</i>-Methyl-<i>L</i>-Tyrosine and its synthetase. 
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<partinfo>BBa_K1976020 short</partinfo>
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            <b>Figure 1:</b>Dimer of the <i>Methanocaldococcus jannaschii</i> tyrosyl-tRNA synthetase specific for <i>O</i>-methyl-<span style="font-variant:small-caps">l</span>-tyrosine
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This Part encodes the tRNA for nonnatural aminoacid <i>O</i>-Methyl-<i>L</i>-Tyrosine and its synthetase.
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===<h2>Usage</h2>===
  
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===Usage and Biology===
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The orthogonal pair for amber supression consists of the tRNA with an anticodon complementary to the amber stop codon and an aminoacyl RNA synthetase (<a href="https://parts.igem.org/Part:BBa_K1976021">BBa_K1976021</a>) specifically loading the tRNA with the non-natural amino acid.
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The occurence of <i>O</i>-methyl-<i>L</i>-tyrosine does not stop the translation of the immunoprotein.
  
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<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
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<partinfo>BBa_K1976020 parameters</partinfo>
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<h2>Sequence and Features</h2>
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<partinfo>BBA_K1976020 SequenceAndFeatures</partinfo>
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<b>References</b>
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Revision as of 10:21, 22 October 2016

O-Methyl-L-tyrosine tRNA synthetase

This Part encodes the tRNA for nonnatural aminoacid O-Methyl-L-Tyrosine and its synthetase.

Figure 1:Dimer of the Methanocaldococcus jannaschii tyrosyl-tRNA synthetase specific for O-methyl-l-tyrosine


Usage

The orthogonal pair for amber supression consists of the tRNA with an anticodon complementary to the amber stop codon and an aminoacyl RNA synthetase (BBa_K1976021) specifically loading the tRNA with the non-natural amino acid. The occurence of O-methyl-L-tyrosine does not stop the translation of the immunoprotein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References