Difference between revisions of "Part:BBa K2130000"
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For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta. | For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta. | ||
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https://static.igem.org/mediawiki/2016/a/a2/T--NTU-Singapore--ruvc3delresult.png | https://static.igem.org/mediawiki/2016/a/a2/T--NTU-Singapore--ruvc3delresult.png | ||
Revision as of 04:44, 22 October 2016
No part name specified with partinfo tag.
dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta.
Sequence and Features No part name specified with partinfo tag.