Difference between revisions of "Part:BBa K1943013:Design"

(→‎Source)
(→‎References)
 
Line 14: Line 14:
  
 
===References===
 
===References===
 +
#[[About_Assembly|Parts Registry Assembly Help]]
 +
#[[Help:Protocols/Transformation|Parts Registry Transformation Guideline]]

Latest revision as of 03:28, 22 October 2016


Puro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


Design Notes

This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. We design primers and do PCR of this anti-antibiotics genes coding sequence and ligate it to pSB1C3 backbone.

Source

The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline