Difference between revisions of "Part:BBa K1943011:Design"
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Revision as of 03:28, 22 October 2016
msfGFP+B0015, green fluorescence protein reporter system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 74
Design Notes
In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. Some of our backbones contain KpnI site, and we need a single KpnI site, while sfGFP contains a KpnI site. So we do site-directed mutagenesis of it. And then we design primer and do PCR of this msfGFP coding device and ligate it to BBa_B0015 (adding a terminator) that we do double enzyme digestion, EcoRI & XbaI.
Source
sfGFP coding sequence was our instructor, Huangwei's lab.
The following list is the Biobricks we’ve used in construction.
1.BBa_B0015