Difference between revisions of "Part:BBa K1943017:Design"

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===Source===
 
===Source===
  
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The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.
  
 
===References===
 
===References===

Revision as of 03:16, 22 October 2016


Puro+T2A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


Design Notes

This year we use mammalian cells as carriers. So in our plasmids, there are several kinds of anti-antibiotics genes which are used for screening. Usually, we will not use anti-antibiotics genes individually. We often use them together with some core protein such as GECO. However, it is monocistronic in the eukaryotic cells. There is a DNA sequence called 2A which connects two coding sequence and then both two call be expressed by the same promoter. We design primers and do PCR of this anti-antibiotics genes coding sequence together with 2A sequence and ligate it to pSB1C3 backbone.

Source

The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.

References