Difference between revisions of "Part:BBa K1943011:Design"

(→‎Design Notes)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
msGFP+B0015
+
sfGFP coding sequence was our instructor, Huangwei's lab.
 +
The following list is the Biobricks we’ve used in construction.<br>
 +
1.<partinfo>BBa_B0015</partinfo>
  
 
===References===
 
===References===

Revision as of 02:48, 22 October 2016


msfGFP+B0015, green fluorescence protein reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. Some of our backbones contain KpnI site, and we need a single KpnI site, while sfGFP contains a KpnI site. So we do site-directed mutagenesis of it. And then we design primer and do PCR of this msfGFP coding device and ligate it to BBa_B0015 (adding a terminator) that we do double enzyme digestion, EcoRI & XbaI.

Source

sfGFP coding sequence was our instructor, Huangwei's lab. The following list is the Biobricks we’ve used in construction.
1.BBa_B0015

References