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| | ===Applications of BBa_K2077000=== | | ===Applications of BBa_K2077000=== |
| − | [[File:T--RHIT--Psb416GPDTuMasterPlate2.png|200px|thumb|left|Figure 1. BY4741 yeast transformants with pSB416 GPD with mRPS12 TU as insert plated on CSM-URA and grown for five days.]]
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| − | [[File:T--RHIT--KnockOutInitialTest.png|200px|thumb|left|Figure 2. BY4741 yeast without pSB416-GPD plated on CSM-URA and CSM-His media. Picture was taken after five days of incubation.]]
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| − | [[File:T--RHIT--FluorsenceComparisonpicture.PNG|200px|thumb|left|Figure 3. Top Left: BY4147 under fluorescent light. Top Right BY4147 under Normal light. Bottom Left: BY4741 with insert under fluorescent light. Bottom Right: BY4741 with insert under normal light]]
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| − | We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. BY4741 yeast that have their URA3 and HIS genes knocked out transformed with pSB416-GPD regained the ability to grow on CSM—URA as shown in Figures 1 & 2. Figure 3 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 00:45, 22 October 2016
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Applications of BBa_K2077000
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