Difference between revisions of "Part:BBa K2128000"

Revision as of 20:56, 21 October 2016

MAHS

This part is a member is a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components.

Preliminary testing of the MAHS LEA protein (expressed using the part K2128200) does not show an improvement in desiccation survivability as shown in Table 1 and Figure 1.

Data beyond H=7 hours of desiccation had failed positive controls that invalidated those results. (Positive controls for hours H=0, 4 and 7 were successful.) While those control issues could not be resolved in time for iGEM deadlines, it was desired to supply this preliminary information as a guide to other teams that may wish consider use of this part.

See team Genspaces 2016 wiki for more information. (http://2016.igem.org/Team:Genspace/Results)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 447
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part is a member is a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components.
+Preliminary testing of the MAHS LEA protein (expressed using the part K2128200) does not show an improvement in desiccation survivability as shown in Table 1 and Figure 1.
+Data beyond H=7 hours of desiccation had failed positive controls that invalidated those results. (Positive controls for hours H=0, 4 and 7 were successful.) While those control issues could not be resolved in time for iGEM deadlines, it was desired to supply this preliminary information as a guide to other teams that may wish consider use of this part.
+See team Genspaces 2016 wiki for more information. (http://2016.igem.org/Team:Genspace/Results)
 <!-- Add more about the biology of this part here