Difference between revisions of "Part:BBa K1943016"

Revision as of 17:53, 21 October 2016

Bla+T2A

In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.

The gene bla express Blasticidin S deaminase. This gene is from Asepergillus terreus. It can inactivate blasticidin S HCl, the nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. We commonly use 10μg/mL in cell line selection.

blasticidin S + H2O ⇌ deaminohydroxyblasticidin S + NH3

Blasticidin: 10μg/ml

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K1943016 short</partinfo>
+In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.
+The gene bla express Blasticidin S deaminase. This gene is from Asepergillus terreus. It can inactivate blasticidin S HCl, the nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. We commonly use 10μg/mL in cell line selection.
+'''blasticidin S + H2O ⇌ deaminohydroxyblasticidin S + NH3'''
 [[File:bla from 2016Sustech.jpeg]]