Difference between revisions of "Part:BBa K1943015"
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The gene bla express Blasticidin S deaminase. This gene is from Asepergillus terreus. It can inactivate blasticidin S HCl, the nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. We commonly use 10μg/mL in cell line selection. | The gene bla express Blasticidin S deaminase. This gene is from Asepergillus terreus. It can inactivate blasticidin S HCl, the nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. We commonly use 10μg/mL in cell line selection. | ||
| − | |||
| − | '''S + H2O ⇌ deaminohydroxyblasticidin S + NH3''' | + | |
| + | '''blasticidin S + H2O ⇌ deaminohydroxyblasticidin S + NH3''' | ||
[[File:bla from 2016Sustech.jpeg]] | [[File:bla from 2016Sustech.jpeg]] | ||
Revision as of 17:52, 21 October 2016
Bla
In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.
The gene bla express Blasticidin S deaminase. This gene is from Asepergillus terreus. It can inactivate blasticidin S HCl, the nucleoside antibiotic isolated from Streptomyces griseochromogenes which inhibits protein synthesis in both prokaryotic and eukaryotic cells. We commonly use 10μg/mL in cell line selection.
blasticidin S + H2O ⇌ deaminohydroxyblasticidin S + NH3
Blasticidin: 10μg/ml
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]