Difference between revisions of "Part:BBa K1983000:Design"
(→‎Primers)
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Primers used for amplification of the fragment: <br>
 
Primers used for amplification of the fragment: <br>
 +
 
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG <br>
 
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG <br>
 
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG <br>
 
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG <br>

Revision as of 14:28, 21 October 2016


Codon optimized AvPAL with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1265
    Illegal XhoI site found at 1702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:

PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG

Primers used for colony PCR screening:

For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC

For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG

Primers used for cloning into biobrick:

PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg
PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG

Source

E coli

References