Difference between revisions of "Part:BBa K1983018:Design"
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VR: attaccgcctttgagtgagc <br> | VR: attaccgcctttgagtgagc <br> | ||
− | Primers used for cloning a | + | Primers used for cloning a low strength RBS: <br> |
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC <br> | RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC <br> | ||
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG <br> | RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG <br> | ||
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===Source=== | ===Source=== |
Revision as of 14:25, 21 October 2016
PheP under constitutive promoter, low strength RBS and terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 98 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Primers used for cloning a low strength RBS:
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG
Source
BBa_K1983018