Difference between revisions of "Part:BBa K1983016:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | ||
+ | ===Primers=== | ||
+ | |||
+ | Primers used for amplification of the fragment: <br> | ||
+ | Uni-FW: GTAGAATTCGCGGCCGCTTCTAG <br> | ||
+ | Uni-RV: GTAGACTGCAGCGGCCGCTACTAG <br> | ||
+ | |||
+ | Primers used for colony PCR screening: <br> | ||
+ | |||
+ | For pSB1C3: <br> | ||
+ | VF2: tgccacctgacgtctaagaa <br> | ||
+ | VR: attaccgcctttgagtgagc <br> | ||
Revision as of 14:20, 21 October 2016
PheP under constitutive promoter and tRNA-Phe under DH10B promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 98 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Source
BBa_K1983016