Difference between revisions of "Part:BBa K2127002:Design"

 
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===Source===
 
===Source===
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The psbB gene codes for the CP-47 subunit of the Cyanobacterial Photosystem II in Synechocystis sp. PCC 6803. Using site-directed Mutagenesis Dr Frankel’s team developed a Synechocystis sp. PCC 6803 mutant containing a histidine tag at the C-Terminus of CP47 subunit designated mutant HT-3.
  
 
NCBI Reference Sequence: NC_000911.1
 
NCBI Reference Sequence: NC_000911.1
  
 
===References===
 
===References===

Revision as of 13:47, 21 October 2016


CP47 His-tagged with Lac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 597
    Illegal AgeI site found at 1194
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918
    Illegal SapI site found at 1595


Design Notes

Double Stop Codon. ORF Check


Source

The psbB gene codes for the CP-47 subunit of the Cyanobacterial Photosystem II in Synechocystis sp. PCC 6803. Using site-directed Mutagenesis Dr Frankel’s team developed a Synechocystis sp. PCC 6803 mutant containing a histidine tag at the C-Terminus of CP47 subunit designated mutant HT-3.

NCBI Reference Sequence: NC_000911.1

References