Difference between revisions of "Part:BBa K1983000:Design"
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===Primers=== | ===Primers=== | ||
| − | Primers used for amplification of the fragment: | + | Primers used for amplification of the fragment: <br> |
| − | PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG | + | PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG <br> |
| − | PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG | + | PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG <br> |
| − | Primers used for colony PCR screening: | + | Primers used for colony PCR screening: <br> |
| − | For pBAD: | + | For pBAD: <br> |
| − | Bad-P1: AGATTAGCGGATCCTACCTGACGC | + | Bad-P1: AGATTAGCGGATCCTACCTGACGC <br> |
| − | Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC | + | Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC <br> |
| − | For pETDuet-1: | + | For pETDuet-1: <br> |
| − | Up-A1: GGATCTCGACGCTCTCCCT | + | Up-A1: GGATCTCGACGCTCTCCCT <br> |
| − | Down3: ACCCCTCAAGACCCGTTTAG | + | Down3: ACCCCTCAAGACCCGTTTAG <br> |
===Source=== | ===Source=== | ||
Revision as of 13:03, 21 October 2016
Codon optimized AvPAL with C-terminal 6XHis-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1265
Illegal XhoI site found at 1702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG
Primers used for colony PCR screening:
For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC
For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG
Source
E coli