Difference between revisions of "Part:BBa J61149"
 
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pBca1101 is a pUC/Amp plasmid in BBb format.  It deviates from pBca9145 by inserting an RFP cassette between the BamHI and XhoI sites.  When a RIPS-generating part is placed between the BglII and BamHI sites, it acts as an RFP reporter.
 
pBca1101 is a pUC/Amp plasmid in BBb format.  It deviates from pBca9145 by inserting an RFP cassette between the BamHI and XhoI sites.  When a RIPS-generating part is placed between the BglII and BamHI sites, it acts as an RFP reporter.
  
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An annotated version of the sequence is [[Arkin_JCA-pBca1101map | here]].
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:37, 5 September 2007

pBca1101 RIPS reporter plasmid in BBb

pBca1101 is a pUC/Amp plasmid in BBb format. It deviates from pBca9145 by inserting an RFP cassette between the BamHI and XhoI sites. When a RIPS-generating part is placed between the BglII and BamHI sites, it acts as an RFP reporter.

An annotated version of the sequence is here. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2905
    Illegal BamHI site found at 1
    Illegal XhoI site found at 851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2905
    Illegal PstI site found at 857
    Illegal AgeI site found at 561
    Illegal AgeI site found at 673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1944