Difference between revisions of "Part:BBa K1954006"
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===Usage and Biology===
 
===Usage and Biology===
  
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This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.
 
This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1954006 parameters</partinfo>
 
<partinfo>BBa_K1954006 parameters</partinfo>
 
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Revision as of 10:32, 21 October 2016


Feedback-resistant N-acetylglutamate synthetase

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 417
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 682


Usage and Biology

N-acetylglutamate synthetase (NAGS) catalyzes the first step of the linear biosynthetic pathway of L-arginine as follows:

acetyl-coA + L-glutamate = coA + N-acetyl-L-glutamate

This sequence is the open reading frame of the feedback resistant mutant PT2M217 NAGS which is not responsive to the inhibition by L-arginine present in the endogenous system.


Functional Parameters