Difference between revisions of "Part:BBa K1992007"

Latest revision as of 10:30, 21 October 2016

PctA-Tar expression system (promoter+RBS+coding+terminator)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 331
Illegal NgoMIV site found at 412
Illegal NgoMIV site found at 416
Illegal NgoMIV site found at 435
Illegal AgeI site found at 535
Illegal AgeI site found at 746
Illegal AgeI site found at 1601
1000
COMPATIBLE WITH RFC[1000]

Introduction

PctA is a chemoreceptor found in the bacterium Pseudomonas Aeruginosa which mediates chemotaxis toward amino acids and away from organic compounds. It is sensitive to all amino acids except for Aspartate (1). A PctA-Tar chimera was formed by the replacement of the Tar LBD with the one of PctA. A procedure which previously was conducted in the literature was recovered (1), in order to prove the concept of the S. Tar platform.

Usage and Biology

Essentially, the expression of the PctA-Tar(BBa_K1992001) chimera receptor is enabled independently by this device, while taking advantage of the bacterium expression resources.

Experiments and results

For methods and results please go to PctA-Tar (BBa_K1992001) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page

@@ Line 7: / Line 7: @@
 <!-- ######################## INTRODUCTION ######################### -->
 ==Introduction==
-Here is the plasmid which serves as the plasmid of the PctA-Tar chimera. The plasmid is comprised of a promoter, a strong RBS , the chimera and a double terminator.
+PctA is a chemoreceptor found in the bacterium Pseudomonas Aeruginosa which mediates chemotaxis toward amino acids and away from organic compounds. It is sensitive to all amino acids except for Aspartate (1).
+A PctA-Tar chimera was formed by the replacement of the Tar LBD with the one of PctA. A procedure which previously was conducted in the literature was recovered (1), in order to prove the concept of the S. Tar platform.
 <!-- ######################## Usage and Biology ######################### -->
 ==Usage and Biology==
-Essentially, the expression of the chimera is enabled independently by this device, while taking advantage of the bacterium expression resources.
+Essentially, the expression of the PctA-Tar(<partinfo>K1992001</partinfo>) chimera receptor is enabled independently by this device, while taking advantage of the bacterium expression resources.
-For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>)
+==Experiments and results==
+For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page
 <!-- Uncomment this to enable Functional Parameter display