Difference between revisions of "Part:BBa K1992009"

 
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<partinfo>BBa_K1992009 short</partinfo>
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<partinfo>BBa_K1992008 short</partinfo>
  
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This device expresses Tar fused with GFP
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1992008 SequenceAndFeatures</partinfo>
  
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==Introduction==
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===Usage and Biology===
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A GFP gene was fused to the improved expression system of the Tar chemoreceptor(<partinfo>K1992006</partinfo>. The plasmid is comprised of a promoter, a strong RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes.
  
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==Usage and Biology==
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<span class='h3bb'>Sequence and Features</span>
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This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.
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<partinfo>BBa_K1992009 SequenceAndFeatures</partinfo>
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==Experiments and results==
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Expariments and results of this exprassion system can be seen in the Tar-GFP part (<partinfo>K1992003</partinfo>) and in our result page [http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation]
  
 
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Revision as of 10:00, 21 October 2016


Tar GFP tagged, native RBS expression system (promoter+RBS+coding+terminator)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2397
    Illegal SapI.rc site found at 184

Introduction

A GFP gene was fused to the improved expression system of the Tar chemoreceptor(BBa_K1992006. The plasmid is comprised of a promoter, a strong RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes.

Usage and Biology

This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.

Experiments and results

Expariments and results of this exprassion system can be seen in the Tar-GFP part (BBa_K1992003) and in our result page [http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation]