Difference between revisions of "Part:BBa K1992008"
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==Introduction== | ==Introduction== | ||
| − | A GFP gene was fused to the improved expression system of the Tar chemoreceptor(<partinfo>K1992005</partinfo>. The plasmid is comprised of a promoter, a native RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes. | + | A GFP gene was fused to the improved expression system of the Tar chemoreceptor(<partinfo>K1992005</partinfo>). The plasmid is comprised of a promoter, a native RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes. |
==Usage and Biology== | ==Usage and Biology== | ||
Revision as of 09:58, 21 October 2016
Tar GFP tagged, native RBS expression system (promoter+RBS+coding+terminator)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1355
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2397
Illegal SapI.rc site found at 184
Introduction
A GFP gene was fused to the improved expression system of the Tar chemoreceptor(BBa_K1992005). The plasmid is comprised of a promoter, a native RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP genes.
Usage and Biology
This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.
Experiments and results
Expariments and results of this exprassion system can be seen in the Tar-GFP part (BBa_K1992003) and in our result page [http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation]