Difference between revisions of "Part:BBa K1996000"
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Putative promoter for suspected ethylene-oxidising genes sourced from Mycobacterium NBB4 | Putative promoter for suspected ethylene-oxidising genes sourced from Mycobacterium NBB4 | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part was obtained from the ethylene metabolism operon in <i>Mycobacterium</i> NBB4. It is thought to be a promoter that regulates bidirectional transcription of gene involved in ethylene metabolism (Figure 1). The enzymes required for ethylene metabolism are contained within an operon organised in two bidirectional gene clusters. One gene cluster encodes the catabolic enzymes required to convert ethylene into acetyl-CoA, the final product of the degradation pathway which is subsequently directed to the tricarboxylic acid cycle. The other gene cluster encodes the enzymes responsible for synthesising coenzyme M, a cofactor required for the function of the second enzyme in the metabolic pathway. | ||
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| + | https://static.igem.org/mediawiki/2016/3/34/T--Sydney_Australia--gene_cluster.png<br> | ||
| + | Figure 1. Schematic of the ethylene metabolism operon in Mycobacterium NBB4. The two bidirectional gene clusters are indicated by the curved brackets. Between the two clusters is the putative promoter for their transcription, EtnP. | ||
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Revision as of 09:43, 21 October 2016
EtnP-Putative promoter region for ethylene oxidation genes in Mycobacterium NBB4
Putative promoter for suspected ethylene-oxidising genes sourced from Mycobacterium NBB4
Usage and Biology
This part was obtained from the ethylene metabolism operon in Mycobacterium NBB4. It is thought to be a promoter that regulates bidirectional transcription of gene involved in ethylene metabolism (Figure 1). The enzymes required for ethylene metabolism are contained within an operon organised in two bidirectional gene clusters. One gene cluster encodes the catabolic enzymes required to convert ethylene into acetyl-CoA, the final product of the degradation pathway which is subsequently directed to the tricarboxylic acid cycle. The other gene cluster encodes the enzymes responsible for synthesising coenzyme M, a cofactor required for the function of the second enzyme in the metabolic pathway.
Figure 1. Schematic of the ethylene metabolism operon in Mycobacterium NBB4. The two bidirectional gene clusters are indicated by the curved brackets. Between the two clusters is the putative promoter for their transcription, EtnP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]