Difference between revisions of "Part:BBa K1961002"

 
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The RecO protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.
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The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.
  
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'''Background '''<br>
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RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).
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[[File:The FOR pathway and RecA filament in the process of SOS response.jpeg|400px|thumb|left|alt text]]
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'''Refernce '''<br>
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Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:45, 21 October 2016


RecO protein-SOS mediator protein

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.


Background

RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).


alt text


Refernce
Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]