Difference between revisions of "Part:BBa K1955006"
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OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA. | OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA. | ||
<br><br> | <br><br> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/2/2d/CGU_Taiwan--bio6.jpg" width=550px height=600px style="border:2px black solid;border-radius:8px;"></img><br><br> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/4/42/J4.jpeg" width=700px height=550px style="border:2px black solid;border-radius:8px;"></img><br><br> | ||
</div> | </div> | ||
</body> | </body> | ||
Revision as of 05:37, 21 October 2016
ppSB1C3-5'HYG-OVA-3'UTR
OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1458
Illegal BamHI site found at 3562 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2689