Difference between revisions of "Part:BBa K1907005"

Revision as of 18:05, 20 October 2016

CCP1 promoter for S. cerevisiae

Introduction

CCP1 is a gene for cytochrome-c peroxidase. Cytochrome-c peroxidase is an enzyme that degrades reactive oxygen species in mitochondria. It is also involved in the response to cell’s oxidative stress. As Ccp1p is produced under oxidative stress, its promoter is activated in these conditions. (Saccharomyces genome database ID: S000001774)

This part contains the promoter region (756 bp) of the CCP1 gene. The length of promoter region is chosen to be the same as previously used by He et al. (2005). This promoter length contains all identified Skn7 and Yap1 binding sites and doesn’t overlap with the next gene in the genome. Skn7 and Yap1 are the main transcription factors activated in oxidative stress and are thus responsible for CCP1 promoter activation. The gene sequence for the CCP1 promoter region is obtained from strain S288C of Saccharomyces cerevisiae, from the Saccharomyces Genome Database.

The functionality of this promoter has been tested in association with the Venus YFP reporter in S. cerevisiae strain SS328-leu, and proven to be functional when oxidative stress is induced by hydrogen peroxide. The CCP1 promoter is hardly expressed in a normal state, and although the expression levels don’t rise very high when induced, the difference in expression is very significant.