Difference between revisions of "Part:BBa K1907004"
 
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Please find the composite part of TSA1 promoter part and Venus YFP part, with which we studied the behaviour of our promoter  from here:
 
Please find the composite part of TSA1 promoter part and Venus YFP part, with which we studied the behaviour of our promoter  from here:
 
https://parts.igem.org/Part:BBa_K1907007
 
https://parts.igem.org/Part:BBa_K1907007
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For more details on the experiments done with this BioBrick, visit http://2016.igem.org/Team:Aalto-Helsinki/Laboratory
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Latest revision as of 18:01, 20 October 2016


TSA1 promoter for S. cerevisiae


Introduction

TSA1 is a gene for thioredoxin peroxidase - a protein that acts as both a ribosome-associated and a free cytoplasmic antioxidant. It self-associates to form a high-molecular weight chaperone complex under oxidative stress. As Tsa1p is produced under oxidative stress, its promoter is activated in these conditions. (Saccharomyces genome database ID: S000004490)

This part contains a promoter region (260 bp) of TSA1 gene. The length of promoter region is chosen so that it contains identified Skn7 and Yap1 binding sites and doesn’t overlap with the next gene in the genome. (He et al., 2005; SGD ID:S000004490) Skn7 and Yap1 are the main transcription factors activated in oxidative stress and are thus responsible for TSA1 promoter activation. The gene sequence for the TSA1 promoter region is taken from strain S288C of Saccharomyces cerevisiae, obtained from the Saccharomyces Genome Database.

The functionality of this promoter has been tested in association with the Venus YFP reporter in S. cerevisiae strain SS328-leu, and proven to be functional when oxidative stress is induced by hydrogen peroxide. The TSA1 promoter has a relatively high base level of expression, but expression levels are further driven up when induced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Please find the composite part of TSA1 promoter part and Venus YFP part, with which we studied the behaviour of our promoter from here: https://parts.igem.org/Part:BBa_K1907007



For more details on the experiments done with this BioBrick, visit http://2016.igem.org/Team:Aalto-Helsinki/Laboratory



References

He, X.J. and Fassler, J.S., 2005. Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae. Molecular microbiology, 58(5), pp.1454-1467.